本刊首页 > 期刊目次 > 气体信号分子与支气管哮喘的相关性研究

• 文章 •

气体信号分子与支气管哮喘的相关性研究

  • 张志海,陈嘉馨,李映梅,谭慧锋

* 通信作者: 陈嘉馨, 单位:广东省佛山南海区第二人民医院 528251

摘要


目的:观察支气管哮喘患者血浆硫化氢(H2S)、一氧化氮(NO)水平的变化,研究其与支气管哮喘的相关性。

方法:70例支气管哮喘分为哮喘缓解期组30例,轻度急性发作期组10例,中度急性发作期组12例,重度急性发作期组18例和正常对照组 15 例,采用去蛋白法紫外可见分光光度测定血浆H2S水平、硝酸还原酶法测定血浆NO水平并检测肺功能和测定诱导痰细胞的计数和分类。

结果:支气管哮喘患者的血浆H2S水平显著低于正常对照组,其中急性发作期组显著低于缓解期组(P < 0.01),急性发作期组中轻度、中度、重度三者之间比较差异均有统计学意义(P <0.01);NO水平显著高于正常对照组(P< 0.01),其中急性发作期组显著高于缓解期组(P < 0.01),急性发作期组中轻度、中度、重度三者之间比较差异均有统计学意义(P < 0.01)。线性相关性分析显示:H2S、NO水平与支气管哮喘发作有相关性。研究对象所处的状态 (健康、 哮喘缓解期及哮喘发作期 )与血浆H2S、NO水平明显相关(r = 0.723、0.786, P < 0.01)。急性发作期患者血浆H2S水平与 FEV1.0 %预 计值( r = 0.845, P < 0.01)和诱导痰巨噬细胞百分比( r = 0.816, P < 0.01)呈正相关。与诱导痰细胞总数、中性粒细胞百分比及诱导痰嗜酸细胞百分比呈负相关(r分别为–0.406、–0.892和-0.638, P < 0.01);急性发作期患者血浆NO水平与 FEV1.0 %预计值( r =-0.872, P < 0.01)和诱导痰巨噬细胞百分比( r = -0.820, P < 0.01)呈负相关;与诱导痰细胞总数、中性粒细胞百分比及诱导痰嗜酸细胞百分比呈正相关 (r分别为0.857、0.816和0.825, P < 0.01)。

结论:患者血浆H2S水平降低、NO水平升高,与支气管哮喘发生、发展相关,可能参与哮喘的发病,内源性H2S、NO作为一种无创性指标监测疾病的严重程度和活动度具有一定意义。

关键词:硫化氢;一氧化氮;哮喘;诱导痰

ABSTRACT


Objective: To investigate the levels of endogenous hydrogen sulfide (H2S) and Nitric oxide(NO) in patients with asthma, and their relativity with asthma.

Methods: Seventy asthmatics were enrolled in this study, thirty cases were clinical relieve, ten cases were light acute exacerbation, twelve cases were mild acute exacerbation, and eighteen cases were severe acute exacerbation, fifteen controls were observed at the same time. We measured the levels of serum H2S, NO, lung function and cell differential count in induced sputum.

Results: There were statistically significant between H2S and NO in light, mild and severe acute exacerbation asthmatics. Concentrations of H2S in asthmatics were significantly lower than those in controls. concentrations of NO in asthmatics were significantly higher than those in controls. All asthmatics and controls had positive correlations with concentrations of H2S and NO(r = 0.723、0.786, P < 0.01).There were positive correlations between concentrations of H2S in acute exacerbation asthmatics with FEV1/pre and percentage of sputum macophages.( r = 0.845, P < 0.01; r = 0.816, P< 0.01), negative correlations between concentrations of H2S in acute exacerbation asthmatics with total count of sputum cells, percentage of sputum neutrophils and percentage of sputum eosinophiles (r = -0.406, P <0.01; r = - 0.892, P <0.01; r = -0.638, P<0.01) .There were negative correlations between concentrations of NO in acute exacerbation asthmatics with FEV1/pre and percentage of sputum macophages ( r = -0.872, P< 0.01; r =-0.820, P< 0.01), positive correlations between concentrations of NO in acute exacerbation asthmatics with total count of sputum cells, percentage of sputum neutrophils and percentage of sputum eosinophiles (r = 0.857, P < 0.01; r = 0.816, P <0.01; r =0.825, P <0.01).

Conclusion  : Endogenous H2S and NO may be involved in the pathogenesis of airway obstruction in asthma and may be a noninvasive marker of disease activity and severity.

Key words: Hydrogen sulfide;Nitric oxide;Asthma;Induced-sputum

引用本文 / How to Cite This Article

张志海,陈嘉馨,李映梅,谭慧锋.气体信号分子与支气管哮喘的相关性研究[J]. 国际精神病学杂志, , (): -

参考文献