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血晶素对间歇性缺氧大鼠作用的研究

  • 柳广南

* 通信作者: 柳广南, 单位:广西医科大学第二附院 530022

摘要

摘 要
目的:研究血晶素对间歇性缺氧大鼠血压的影响,探讨间歇性缺氧致动物血压变化的机理和调控

方法

方法:雄性Spague-Dawley(SD)大鼠24只,体重250g~300g,鼠龄10~12W。按随机数字表法随机分为实验组(血晶素组,A组)、实验对照组(间歇缺氧组,B组)及空白对照组(正常组,C组),每组8例。血晶素组:大鼠注射血晶素30分钟后置于常压低氧有机玻璃箱内,使其间歇性缺氧8小时/天;间歇缺氧组:大鼠注射生理盐水30分钟后置于常压低氧有机玻璃箱内,使其间歇性缺氧8小时/天,箱体内氧气浓度与血晶素组相同;正常组:正常饲养,不作任何实验干预。3组大鼠均在同等条件下室温常规饲养。35天后测定大鼠平均颈动脉压力,检测其血浆一氧化碳含量、应用逆转录聚合酶链反应检测相关组织器官中HO-1mRNA的表达、用免疫组织化学检测相关组织器官中HO-1表达情况。

结果:①平均颈动脉压力:间歇缺氧组较血晶素组及正常组明显升高(P<0.05);血晶素组较正常组高(P<0.05),较间歇缺氧组低(P<0.05)。②血浆CO含量:血晶素组较间歇缺氧组、正常组高(P<0.05)。间歇缺氧组与正常组比较无统计学差异。(P>0.05)。③肝、脾、肺、肾HO-1mRNA的表达:血晶素组较间歇缺氧组及正常组高(P<0.05),间歇缺氧组较正常组高(P<0.05)。④间歇缺氧组大鼠的平均颈动脉压力与血浆CO含量无相关关系(r =0.399,P=0.327)。血晶素组大鼠的平均颈动脉压力与血浆CO含量呈负相关关系(r =-0.909,P=0.002)。

结论:间歇性低氧可使大鼠的血压显著升高,相关组织器官中的HO-1表达增加,但其生成物CO却无显著增高。而使用HO-1诱导剂血晶素使间歇性缺氧大鼠体内HO-1及CO表达增加,使慢性缺氧所致大鼠高血压有一定程度下降,因此,可以考虑将血晶素用于OSA相关性高血压的治疗。

关键词:血晶素;间歇性低氧; 高血压;内源性一氧化碳;血红素合酶-1

ABSTRACT

Abstract
Objective To study the influence of hemin on the blood pressure of intermittent hypoxic rats and research the its mechanism to evaluate its possibility to treat hypertension caused by intermittent hypoxia .

Methods 24 Sprague-Dawley(SD) male rats were randomly divided into experiment group(group A),control group (group B)and blank group (group C). 30 minutes after injected with hemin, group A was exposed to intermittent normobaric hypoxic environment (8 h/day). Group B was injected with normal sodium and then exposed to the same environment (8 h/day). Group C just inhaled air in the room. The three groups were bred in the same condition. 35 days later, the carotid artery pressure (CAP) of rats was measured and their plasma carbon monoxide(CO)was done by Chalmer’s method. Transcriptase polymerase chain reaction (RT-PCR) was performed to detect the levels of HO-1 mRNA expression in lung, liver, spleen, kidney and other’s rats’ organs .The expression of HO-1 protein in the organs was also detected by immunohistochemistry.

Results ①The mean carotid artery pressure:The mean carotid artery pressure in group B was significantly higher than group A and group C(P<0.05). The mean carotid artery pressure in group A was higher than group C but lower than group B(P<0.05). ②The concentration of plasma CO:Plasma CO in group A was higher than group B and group C(P<0.05). there was no statistics difference of plasma CO between group B and C(P>0.05).③The expression of HO-1 mRNA of lung, liver, spleen and kidney in group A and B was higher than group C(P<0.05).The expression of HO-1 mRNA in group A was also higher than group B (P<0.05). ④There were no relationship between the mean carotid artery pressure and the concentration of plasma CO of kidney in hypoxic group rats(r =0.399,P=0.327). There were significant negative relationship between the mean carotid artery pressure and the concentration of plasma CO and HO-1 mRNA expression of kidney in hemin group rats(r =-0.909,P=0.002).

Conclusion  s Intermittent hypoxia could lead to hypertensive rats. The HO-1 expression was increased in hypoxic rats, but the plasma CO did not have significant increase. The inducer of HO-1(hemin) could increase the expression of HO-1 and CO of the hypoxic rats, then lower their blood pressure caused by intermittent hypoxia to some extent.So ,hemin should be considered to treat hypertension caused by OSA in the futur.

Key words: hemin; intermittent hypoxia; hypertension; endogenous carbon monoxide;heme oxygenase-1

引用本文 / How to Cite This Article

柳广南.血晶素对间歇性缺氧大鼠作用的研究[J]. 国际精神病学杂志, , (): -

参考文献